Changes in mRNA expression of colon mucosa genes during natural aging

Background: during natural aging, cellular and molecular changes in the colonic mucosa contribute to chronic damage development of various neoplasia, including colorectal cancer (CRC). Aim: assess age peculiarities mRNA expression samples normal from parts colon. Patients Methods: study included 95 patients. Normal biopsies were derived videocolonoscopy different colon, i.e., right colon (n=91), left (n=95), rectum (n=87). In total, 273 examined. The relative level 63 genes potentially involved pathways CRC carcino-genesis was evaluated by reverse transcription polymerase chain reaction. Results: a linear regression model for predicting passport age, data on gene ex-pression, characterized moderate reliability (R2 0.45, DW test 1.54). Age estimation er-rors arise proportionally its increase, which is associated with accumulation epigenetic changes. Maximum accuracy prediction typical rectal samples. first statistically significant division (primary classification node) patients indi-viduals over or under 60 years provides TERT expression. Telomerase activity association HLA-G5 overexpression older patients, whereas hypoexpression simultaneous granulysin (GNLY) are younger than years. A cascade involving decrease telomerase due cell cycle regulator tumor suppressor P14ARF also aged over. Conclusion: our findings support genetic that occur age. specifics carcinogenesis basis assessing risk. KEYWORDS: expression, mRNA, RT-PCR, mucosa, changes, cancer. FOR CITATION: Goncharov S.V., Bozhenko V.K., Zakharenko M.V., Chaptykov A.A., Kulinich T.M., Solodkiy V.A. Changes aging. Russian Medical Inquiry. 2023;7(6):347–352 (in Russ.). DOI: 10.32364/2587-6821-2023-7-6-2.